WB
Western blot analysis of LRP8 using anti-LRP8 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U87 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human U937 whole cell lysates, Lane 5: human HL-60 whole cell lysates, Lane 6: human A431 whole cell lysates, Lane 7: human HELA whole cell lysates, Lane 8: rat testis tissue lysates, Lane 9: rat thymus tissue lysates, Lane 10: rat spleen tissue lysates, Lane 11: rat heart tissue lysates, Lane 12: mouse testis tissue lysates, Lane 13: mouse thymus tissue lysates, Lane 14: mouse heart tissue lysates, Lane 15: Mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LRP8 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of LRP8 using anti-LRP8 antibody. LRP8 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-LRP8 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of LRP8 using anti-LRP8 antibody. LRP8 was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-LRP8 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of LRP8 using anti-LRP8 antibody. LRP8 was detected in a paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-LRP8 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
IF analysis of LRP8 using anti-LRP8 antibody. LRP8 was detected in an immunocytochemical section of HepG2 cells. The section was incubated with rabbit anti-LRP8 Antibody at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of U87 cells using anti-LRP8 antibody. Overlay histogram showing U87 cells stained with anti-LRP8 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LRP8 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to LRP8 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human ApoER2/LRP8 recombinant protein (Position: R444-D960). Human LRP8 shares 92.3% and 90.7% amino acid (aa) sequence identity with mouse and rat LRP8, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LRP8 |
|---|---|
| Gene Synonyms | MCI1 LRP-8 APOER2 HSZ75190 |
| Gene Full Name | LDL receptor related protein 8 |
| Gene Summary | This gene encodes a member of the low density lipoprotein receptor (LDLR) family. Low density lipoprotein receptors are cell surface proteins that play roles in both signal transduction and receptor-mediated endocytosis of specific ligands for lysosomal degradation. The encoded protein plays a critical role in the migration of neurons during development by mediating Reelin signaling, and also functions as a receptor for the cholesterol transport protein apolipoprotein E. Expression of this gene may be a marker for major depressive disorder. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jun 2011] |
| Alternative Names | LDL receptor related protein 8 APOER2 HSZ75190 LRP-8 MCI1 LRP8 |
| Molecular Weight(MW) | 106kDa |
| Cellular Localization | Cell membrane,Secreted. |
WB
Western blot analysis of LRP8 using anti-LRP8 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U87 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human U937 whole cell lysates, Lane 5: human HL-60 whole cell lysates, Lane 6: human A431 whole cell lysates, Lane 7: human HELA whole cell lysates, Lane 8: rat testis tissue lysates, Lane 9: rat thymus tissue lysates, Lane 10: rat spleen tissue lysates, Lane 11: rat heart tissue lysates, Lane 12: mouse testis tissue lysates, Lane 13: mouse thymus tissue lysates, Lane 14: mouse heart tissue lysates, Lane 15: Mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LRP8 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of LRP8 using anti-LRP8 antibody. LRP8 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-LRP8 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of LRP8 using anti-LRP8 antibody. LRP8 was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-LRP8 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of LRP8 using anti-LRP8 antibody. LRP8 was detected in a paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-LRP8 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
IF analysis of LRP8 using anti-LRP8 antibody. LRP8 was detected in an immunocytochemical section of HepG2 cells. The section was incubated with rabbit anti-LRP8 Antibody at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of U87 cells using anti-LRP8 antibody. Overlay histogram showing U87 cells stained with anti-LRP8 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LRP8 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
