WB
Western blot analysis of biologically active human recombinant VEGF (A) and mouse recombinant VEGF (B).IHC
Immunoperoxidase staining of human liver tissue fixed in formalin and embedded in paraffin reveals cytoplasmic staining of liver cells.IHC
Immunoperoxidase staining of human thyroid tissue fixed in formalin and embedded in paraffin reveals cytoplasmic staining of glandular cells.IHC
Immunoperoxidase staining of formalin fixed and paraffin embedded human lung tissue reveals cytoplasmic staining of lung cells and macrophages.| Product Name | Mouse Monoclonal antibody to VEGF |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Raised against recombinant VEGF189 protein of human origin |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:100-1:1000 IHC:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VEGFA |
|---|---|
| Gene Synonyms | VPF VEGF MVCD1 L-VEGF |
| Gene Full Name | vascular endothelial growth factor A |
| Gene Summary | This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. The levels of VEGF are increased during infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), thus promoting inflammation by facilitating recruitment of inflammatory cells, and by increasing the level of angiopoietin II (Ang II), one of two products of the SARS-CoV-2 binding target, angiotensin-converting enzyme 2 (ACE2). In turn, Ang II facilitates the elevation of VEGF, thus forming a vicious cycle in the release of inflammatory cytokines. [provided by RefSeq, Jun 2020] |
| Molecular Weight(MW) | Molecular Weight of VEGF monomer: 21 kDa. Molecular Weight of VEGF dimer: 42 kDa. |
WB
Western blot analysis of biologically active human recombinant VEGF (A) and mouse recombinant VEGF (B).
IHC
Immunoperoxidase staining of human liver tissue fixed in formalin and embedded in paraffin reveals cytoplasmic staining of liver cells.
IHC
Immunoperoxidase staining of human thyroid tissue fixed in formalin and embedded in paraffin reveals cytoplasmic staining of glandular cells.
IHC
Immunoperoxidase staining of formalin fixed and paraffin embedded human lung tissue reveals cytoplasmic staining of lung cells and macrophages.| Application Notes | WB:1:100-1:1000 IHC:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
