WB
Western blot analysis of anti- SLC40A1 antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse liver tissue lysates. Use rabbit anti- SLC40A1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit.IHC
IHC analysis of FPN1/SLC40A1 using anti-FPN1/SLC40A1 antibody. FPN1/SLC40A1 was detected in a paraffin-embedded section of human larynx squamous cell carcinoma tissue. The tissue section was incubated with rabbit anti-FPN1/SLC40A1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of FPN1/SLC40A1 using anti-FPN1/SLC40A1 antibody. FPN1/SLC40A1 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-FPN1/SLC40A1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of FPN1/SLC40A1 using anti-FPN1/SLC40A1 antibody. FPN1/SLC40A1 was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. The tissue section was incubated with rabbit anti-FPN1/SLC40A1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF-P
IF analysis of SLC40A1 using anti-SLC40A1 antibody. SLC40A1 was detected in a paraffin-embedded section of human ovarian cancer tissue. FITC Conjugated AffiniPure Goat Anti-mouse IgG (H+L) Secondary Antibody (green) was used as secondary antibody. The section was counterstained with DAPI(Blue).FC
Flow Cytometry analysis of U2OS cells using anti-FPN1/SLC40A1 antibody. Overlay histogram showing U2OS cells stained with FPN1/SLC40A1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FPN1/SLC40A1 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to FPN1/SLC40A1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human SLC40A1 recombinant protein (Position: M1-V571). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC40A1 |
|---|---|
| Gene Synonyms | FPN FPN1 HFE4 MTP1 IREG1 MST079 MSTP079 SLC11A3 |
| Gene Full Name | solute carrier family 40 member 1 |
| Gene Summary | The protein encoded by this gene is a cell membrane protein that may be involved in iron export from duodenal epithelial cells. Defects in this gene are a cause of hemochromatosis type 4 (HFE4). [provided by RefSeq, Jul 2008] |
| Alternative Names | Ferroportin 1 FPN1 HFE4 IREG1 Iron regulated transporter 1 MST079 MSTP079 MTP1 SLC11A3 SLC40A1 |
| Molecular Weight(MW) | 63kDa(Observed MW 70kDa) |
| Cellular Localization | Cell membrane,Basolateral cell membrane. |
WB
Western blot analysis of anti- SLC40A1 antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse liver tissue lysates. Use rabbit anti- SLC40A1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit.
IHC
IHC analysis of FPN1/SLC40A1 using anti-FPN1/SLC40A1 antibody. FPN1/SLC40A1 was detected in a paraffin-embedded section of human larynx squamous cell carcinoma tissue. The tissue section was incubated with rabbit anti-FPN1/SLC40A1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of FPN1/SLC40A1 using anti-FPN1/SLC40A1 antibody. FPN1/SLC40A1 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-FPN1/SLC40A1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of FPN1/SLC40A1 using anti-FPN1/SLC40A1 antibody. FPN1/SLC40A1 was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. The tissue section was incubated with rabbit anti-FPN1/SLC40A1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF-P
IF analysis of SLC40A1 using anti-SLC40A1 antibody. SLC40A1 was detected in a paraffin-embedded section of human ovarian cancer tissue. FITC Conjugated AffiniPure Goat Anti-mouse IgG (H+L) Secondary Antibody (green) was used as secondary antibody. The section was counterstained with DAPI(Blue).
FC
Flow Cytometry analysis of U2OS cells using anti-FPN1/SLC40A1 antibody. Overlay histogram showing U2OS cells stained with FPN1/SLC40A1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FPN1/SLC40A1 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
