WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-A20 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela .IHC
Human kidney was stained with anti-A20 Rabbit antibody.IHC
Human placenta was stained with anti-A20 Rabbit antibody.ICC/IF
Immunofluorescence analysis of HepG2 cells, using A20 Antibody. The picture on the right is blocked with the synthesized peptide.| Product Name | A20 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000-1:10000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TNFAIP3 |
|---|---|
| Gene Synonyms | A20 AISBL AIFBL1 OTUD7C TNFA1P2 |
| Gene Full Name | TNF alpha induced protein 3 |
| Gene Summary | This gene was identified as a gene whose expression is rapidly induced by the tumor necrosis factor (TNF). The protein encoded by this gene is a zinc finger protein and ubiqitin-editing enzyme, and has been shown to inhibit NF-kappa B activation as well as TNF-mediated apoptosis. The encoded protein, which has both ubiquitin ligase and deubiquitinase activities, is involved in the cytokine-mediated immune and inflammatory responses. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2012] |
| Alternative Names | TNFAIP3 OTUD7C Tumor necrosis factor alpha-induced protein 3 TNF alpha-induced protein 3 OTU domain-containing protein 7C Putative DNA-binding protein A20 Zinc finger protein A20 |
| Molecular Weight(MW) | 90kDa(Observed 82kDa) |
| Cellular Localization | Cytoplasm. Nucleus. Lysosome. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-A20 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela .
IHC
Human kidney was stained with anti-A20 Rabbit antibody.
IHC
Human placenta was stained with anti-A20 Rabbit antibody.
ICC/IF
Immunofluorescence analysis of HepG2 cells, using A20 Antibody. The picture on the right is blocked with the synthesized peptide.| Application Notes | WB:1:2000-1:10000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
