WB
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TRMU monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).ICC/IF
Anti-TRMU mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TRMU.| Product Name | TRMT1 (TRMU) Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Full length human recombinant protein of human TRMU(NP_060476) produced in HEK293T cell. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| WB:1:200-1:2000 ICC/IF:1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Gene Symbol | TRMU |
|---|---|
| Gene Synonyms | MTO2 MTU1 TRMT LCAL3 TRMT1 |
| Gene Full Name | tRNA mitochondrial 2-thiouridylase |
| Gene Summary | This nuclear gene encodes a mitochondrial tRNA-modifying enzyme. The encoded protein catalyzes the 2-thiolation of uridine on the wobble positions of tRNA(Lys), tRNA(Glu), and tRNA(Gln), resulting in the formation of 5-taurinomethyl-2-thiouridine moieties. Mutations in this gene may cause transient infantile liver failure. Polymorphisms in this gene may also influence the severity of deafness caused by mitochondrial 12S ribosomal RNA mutations. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] |
| Alternative Names | LCAL3 MTO2 MTU1 TRMT TRMT1 TRNT1 |
| Molecular Weight(MW) | 47kDa |
| Cellular Localization | Mitochondrion. |
WB
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TRMU monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
ICC/IF
Anti-TRMU mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TRMU.| Application Notes | WB:1:200-1:2000 ICC/IF:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C as received. |
| Storage Buffer | PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
