WB
Non-treated and PMA treated THP-1 cellls were subjected to SDS PAGE followed by western blot with TREM2 antibody at dilution of 1:5000 incubated at room temperature for 1 hours.IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TREM2 antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using TREM2 antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Product Name | TREM2 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human TREM2 protein. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IF-PWB |
| WB:1:500-1:2000 IF-P:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TREM2 |
|---|---|
| Gene Synonyms | AD17 PLOSL2 TREM-2 Trem2a Trem2b Trem2c |
| Gene Full Name | triggering receptor expressed on myeloid cells 2 |
| Gene Summary | This gene encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012] |
| Alternative Names | Trem2c Trem2b Trem2a TREM-2 TREM 2 |
| Molecular Weight(MW) | 26-32kDa |
| Cellular Localization | Cell membrane,Secreted. |
WB
Non-treated and PMA treated THP-1 cellls were subjected to SDS PAGE followed by western blot with TREM2 antibody at dilution of 1:5000 incubated at room temperature for 1 hours.
IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TREM2 antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using TREM2 antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Application Notes | WB:1:500-1:2000 IF-P:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
