WB
Western blot analysis of FAM38B / PIEZO2 on different lysates with Rabbit anti-FAM38B / PIEZO2 antibody at 1/2,000 dilution. Lane 1: 293T transfected with mPiezo1 cell lysate (negative), Lane 2: 293T transfected with mPiezo2 cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of 293T cells labeling FAM38B / PIEZO2 with Rabbit anti-FAM38B / PIEZO2 antibody at 1/200 dilution. 293T cells, transfected with GFP-tagged mouse Piezo1 (top, negative) / tag-free mouse Piezo2 (bottom, positive) expression vector, respectively, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FAM38B / PIEZO2 antibody at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.| Product Name | FAM38B / PIEZO2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within mouse PIEZO2 aa 2,497-2,731. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:2000 ICC/IF:1:200 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Piezo2 |
|---|---|
| Gene Synonyms | Fam38b Fam38b2 5930434P17 9030411M15Rik 9430028L06Rik |
| Gene Full Name | piezo-type mechanosensitive ion channel component 2 |
| Gene Summary | Enables mechanosensitive monoatomic ion channel activity and monoatomic cation channel activity. Involved in detection of mechanical stimulus involved in sensory perception and monoatomic cation transport. Acts upstream of or within regulation of membrane potential. Located in cuticular plate; neuronal cell body membrane; and stereocilium. Is expressed in dorsal root ganglion; forelimb; lung; lung epithelium; and thoracic dorsal root ganglion. Human ortholog(s) of this gene implicated in distal arthrogryposis type 3 and distal arthrogryposis type 5. Orthologous to human PIEZO2 (piezo type mechanosensitive ion channel component 2). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | Piezo-type mechanosensitive ion channel component 2 Protein FAM38B Piezo2 Fam38b |
| Molecular Weight(MW) | 326kDa |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of FAM38B / PIEZO2 on different lysates with Rabbit anti-FAM38B / PIEZO2 antibody at 1/2,000 dilution. Lane 1: 293T transfected with mPiezo1 cell lysate (negative), Lane 2: 293T transfected with mPiezo2 cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of 293T cells labeling FAM38B / PIEZO2 with Rabbit anti-FAM38B / PIEZO2 antibody at 1/200 dilution. 293T cells, transfected with GFP-tagged mouse Piezo1 (top, negative) / tag-free mouse Piezo2 (bottom, positive) expression vector, respectively, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FAM38B / PIEZO2 antibody at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.| Application Notes | WB:1:2000 ICC/IF:1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
