WB
Western blot analysis of PLIN5 using anti-PLIN5 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HT1080 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PLIN5 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of PLIN5 using anti-PLIN5 antibody. PLIN5 was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was incubated with rabbit anti-PLIN5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of PLIN5 using anti-PLIN5 antibody. PLIN5 was detected in a paraffin-embedded section of mouse heart tissue. The tissue section was incubated with rabbit anti-PLIN5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of PLIN5 using anti-PLIN5 antibody. PLIN5 was detected in a paraffin-embedded section of rat heart tissue. The tissue section was incubated with rabbit anti-PLIN5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF-P
IF analysis of PLIN5 using anti-PLIN5 antibody. PLIN5 was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was incubated with rabbit anti-PLIN5 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)was used as secondary antibody. The section was counterstained with DAPI(Blue).IF=P
IF analysis of PLIN5 using anti-PLIN5 antibody. PLIN5 was detected in a paraffin-embedded section of human stomach cancer tissue. The tissue section was incubated with rabbit anti-PLIN5 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI(Blue).FC
Flow Cytometry analysis of HepG2 cells using anti-PLIN5 antibody. Overlay histogram showing HepG2 cells stained with anti-PLIN5 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PLIN5 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to PLIN5 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human PLIN5 recombinant protein (Position: M1-D425). Human PLIN5 shares 74.1% and 70.9% amino acid (aa) sequence identity with mouse and rat PLIN5, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PLIN5 |
|---|---|
| Gene Synonyms | MLDP LSDA5 LSDP5 OXPAT |
| Gene Full Name | perilipin 5 |
| Gene Summary | Predicted to enable identical protein binding activity and lipase binding activity. Predicted to be involved in several processes, including negative regulation of peroxisome proliferator activated receptor signaling pathway; positive regulation of triglyceride storage; and regulation of lipid metabolic process. Located in intracellular membrane-bounded organelle and lipid droplet. [provided by Alliance of Genome Resources, Apr 2025] |
| Alternative Names | PLIN5 LSDP5 OXPAT PAT-1 Perilipin-5 Lipid storage droplet protein 5 |
| Molecular Weight(MW) | 55kDa |
| Cellular Localization | Cytoplasm,Lipid droplet,Mitochondrion. |
WB
Western blot analysis of PLIN5 using anti-PLIN5 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HT1080 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PLIN5 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of PLIN5 using anti-PLIN5 antibody. PLIN5 was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was incubated with rabbit anti-PLIN5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of PLIN5 using anti-PLIN5 antibody. PLIN5 was detected in a paraffin-embedded section of mouse heart tissue. The tissue section was incubated with rabbit anti-PLIN5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of PLIN5 using anti-PLIN5 antibody. PLIN5 was detected in a paraffin-embedded section of rat heart tissue. The tissue section was incubated with rabbit anti-PLIN5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF-P
IF analysis of PLIN5 using anti-PLIN5 antibody. PLIN5 was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was incubated with rabbit anti-PLIN5 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)was used as secondary antibody. The section was counterstained with DAPI(Blue).
IF=P
IF analysis of PLIN5 using anti-PLIN5 antibody. PLIN5 was detected in a paraffin-embedded section of human stomach cancer tissue. The tissue section was incubated with rabbit anti-PLIN5 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI(Blue).
FC
Flow Cytometry analysis of HepG2 cells using anti-PLIN5 antibody. Overlay histogram showing HepG2 cells stained with anti-PLIN5 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PLIN5 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
