WB
Western blot analysis of MOB1 on different lysates with Rabbit anti-MOB1 antibody at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane), Lane 2: K-562 cell lysate (20 µg/Lane), Lane 3: PANC-1 cell lysate (20 µg/Lane), Lane 4: MOLT-4 cell lysate (20 µg/Lane), Lane 5: Daudi cell lysate (20 µg/Lane), Lane 6: Raji cell lysate (20 µg/Lane), Lane 7: C2C12 cell lysate (20 µg/Lane), Lane 8: NIH/3T3 cell lysate (20 µg/Lane), Lane 9: RAW264.7 cell lysate (20 µg/Lane), Lane 10: C6 cell lysate (20 µg/Lane), Lane 11: PC-12 cell lysate (20 µg/Lane), Lane 12: COS-1 cell lysate (20 µg/Lane), Lane 13: Mouse pancreas tissue lysate (40 µg/Lane), Lane 14: Rat pancreas tissue lysate (40 µg/Lane), Exposure time: 8 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-MOB1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-MOB1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-MOB1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of RAW264.7 cells labeling MOB1 with Rabbit anti-MOB1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MOB1 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.FC
Flow cytometric analysis of RAW264.7 cells labeling MOB1. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).IP
MOB1 was immunoprecipitated from 0.2 mg RAW264.7 cell lysate with Rabbit anti-MOB1 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-MOB1 antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: RAW264.7 cell lysate (input) Lane 2: Rabbit anti-MOB1 antibody IP in RAW264.7 cell lysate Lane 3: Rabbit IgG instead of Rabbit anti-MOB1 antibody in RAW264.7 cell lysate Blocking/Dilution buffer: primary antibody dilution , Exposure time: 10 seconds.| Product Name | MOB1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human MOB1A aa 1-216. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:5000 IHC:1:1000 ICC/IF:1:50-1:100 FC:1:1000 IP: 1-2μg/sample |
|
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MOB1A |
|---|---|
| Gene Synonyms | MOB1 MATS1 Mob4B C2orf6 MOBK1B MABKL1B MOBKL1B |
| Gene Full Name | MOB kinase activator 1A |
| Gene Summary | The protein encoded by this gene is a component of the Hippo signaling pathway, which controls organ size and tumor growth by enhancing apoptosis. Loss of the encoded protein results in cell proliferation and cancer formation. The encoded protein is also involved in the control of microtubule stability during cytokinesis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015] |
| Alternative Names | B1 Mps One Binder kinase activator like 1B (yeast) antibody C2orf6 antibody Chromosome 2 open reading frame 6 antibody FLJ10788 antibody FLJ11595 antibody MATS1 antibody MOB kinase activator 1A antibody MOB1 antibody Mob1 alpha antibody Mob1 homolog 1B antibody B1 Mps One Binder kinase activator like 1B (yeast) antibody C2orf6 antibody Chromosome 2 open reading frame 6 antibody FLJ10788 antibody FLJ11595 antibody MATS1 antibody MOB kinase activator 1A antibody MOB1 antibody Mob1 alpha antibody Mob1 homolog 1B antibody MOB1 Mps One Binder homolog A antibody MOB1 Mps One Binder kinase activator like 1B antibody Mob1A antibody MOB4B antibody Mob4B protein antibody MOBK1B antibody Mobkl1b antibody MOL1B_HUMAN antibody Mps one binder kinase activator like 1B antibody Mps one binder kinase activator-like 1B antibody Protein Mob4B antibody MOB kinase activator 1B Mob1 homolog 1A Mob1B Mps one binder kinase activator-like 1A MOB1B MOB4A MOBKL1A |
| Molecular Weight(MW) | 25kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
WB
Western blot analysis of MOB1 on different lysates with Rabbit anti-MOB1 antibody at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane), Lane 2: K-562 cell lysate (20 µg/Lane), Lane 3: PANC-1 cell lysate (20 µg/Lane), Lane 4: MOLT-4 cell lysate (20 µg/Lane), Lane 5: Daudi cell lysate (20 µg/Lane), Lane 6: Raji cell lysate (20 µg/Lane), Lane 7: C2C12 cell lysate (20 µg/Lane), Lane 8: NIH/3T3 cell lysate (20 µg/Lane), Lane 9: RAW264.7 cell lysate (20 µg/Lane), Lane 10: C6 cell lysate (20 µg/Lane), Lane 11: PC-12 cell lysate (20 µg/Lane), Lane 12: COS-1 cell lysate (20 µg/Lane), Lane 13: Mouse pancreas tissue lysate (40 µg/Lane), Lane 14: Rat pancreas tissue lysate (40 µg/Lane), Exposure time: 8 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-MOB1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-MOB1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-MOB1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of RAW264.7 cells labeling MOB1 with Rabbit anti-MOB1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MOB1 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of RAW264.7 cells labeling MOB1. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
IP
MOB1 was immunoprecipitated from 0.2 mg RAW264.7 cell lysate with Rabbit anti-MOB1 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-MOB1 antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: RAW264.7 cell lysate (input) Lane 2: Rabbit anti-MOB1 antibody IP in RAW264.7 cell lysate Lane 3: Rabbit IgG instead of Rabbit anti-MOB1 antibody in RAW264.7 cell lysate Blocking/Dilution buffer: primary antibody dilution , Exposure time: 10 seconds.| Application Notes | WB:1:5000 IHC:1:1000 ICC/IF:1:50-1:100 FC:1:1000 IP: 1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
