WB
Western Blot analysis of Jurkat, K562 cells using Acetyl-Ref-1 (K7) Polyclonal Antibody. Secondary antibody was diluted at 1:20000.WB
Western Blot analysis of JK cells using Acetyl-Ref-1 (K7) Polyclonal Antibody. Secondary antibody was diluted at 1:20000.| Product Name | Ref-1 (Acetyl Lys7) Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized Acetyl-peptide derived from human APE1 around the Acetylation site of Lys7. AA range:1-50. |
| Modification | Acetyl Lys7 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | APEX1 |
|---|---|
| Gene Synonyms | APE APX APE1 APEN APEX HAP1 REF1 |
| Gene Full Name | apurinic/apyrimidinic endodeoxyribonuclease 1 |
| Gene Summary | The APEX gene encodes the major AP endonuclease in human cells. It encodes the APEX endonuclease, a DNA repair enzyme with apurinic/apyrimidinic (AP) activity. Such AP activity sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. The AP sites are the most frequent pre-mutagenic lesions that can prevent normal DNA replication. Splice variants have been found for this gene; all encode the same protein. Disruptions in the biological functions related to APEX are associated with many various malignancies and neurodegenerative diseases.[provided by RefSeq, Dec 2019] |
| Target | Acetyl-Ref-1 (K7) Polyclonal Antibody detects endogenous levels of Ref-1 protein only when acetylated at K7.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):RGkKG |
| Alternative Names | APEX1 APE APE1 APEX APX HAP1 REF1 DNA- apurinic or apyrimidinic site lyase APEX nuclease APEN Apurinic-apyrimidinic endonuclease 1 AP endonuclease 1 APE-1 REF-1 Redox factor-1 |
| Molecular Weight(MW) | 35kDa |
| Cellular Localization | Nucleus, Cytoplasm. |
WB
Western Blot analysis of Jurkat, K562 cells using Acetyl-Ref-1 (K7) Polyclonal Antibody. Secondary antibody was diluted at 1:20000.
WB
Western Blot analysis of JK cells using Acetyl-Ref-1 (K7) Polyclonal Antibody. Secondary antibody was diluted at 1:20000.| Application Notes | WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
