WB
Various lysates were subjected to SDS PAGE followed by western blot with SIRT2 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody as loading control.IHC
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using SIRT2 Antibody at dilution of 1:200 (under 10x lens).IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using SIRT2 Antibody at dilution of 1:200 (under 10x lens).ICC/IF
Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using SIRT2 antibody at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).| Product Name | SIRT2 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human SIRT2 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:5000-1:20000 IHC:1:50-1:500 ICC/IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SIRT2 |
|---|---|
| Gene Synonyms | SIR2 SIR2L SIR2L2 |
| Gene Full Name | sirtuin 2 |
| Gene Summary | This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Several transcript variants are resulted from alternative splicing of this gene. [provided by RefSeq, Jul 2010] |
| Alternative Names | SIR2 SIR2 like protein 2 SIR2L SIR2L2 SIRT2 |
| Molecular Weight(MW) | 37-45 kDa |
| Cellular Localization | Cell membrane,Cell projection,Chromosome,Cytoplasm,Cytoskeleton,Membrane,Microtubule,Nucleus. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with SIRT2 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody as loading control.
IHC
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using SIRT2 Antibody at dilution of 1:200 (under 10x lens).
IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using SIRT2 Antibody at dilution of 1:200 (under 10x lens).
ICC/IF
Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using SIRT2 antibody at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).| Application Notes | WB:1:5000-1:20000 IHC:1:50-1:500 ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
