WB
Positive WB detected in: THP-1 whole cell lysate(20µg), K562 whole cell lysate(20µg), JK whole cell lysate(20µg), All lanes: ADAM8 antibody at 1:1000, Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Exposure time: 300s.ICC/IF
Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-ADAM8 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthesized peptide derived from part region of human protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000-1:5000 IHC:1:25-1:100 ICC/IF:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ADAM8 |
|---|---|
| Gene Synonyms | MS2 CD156 CD156a |
| Gene Full Name | ADAM metallopeptidase domain 8 |
| Gene Summary | This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene may be involved in cell adhesion during neurodegeneration, and it is thought to be a target for allergic respiratory diseases, including asthma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2009] |
| Alternative Names | A Disintegrin And Metalloproteinase domain 8 antibody ADAM 8 antibody ADAM 8 precursor antibody ADAM metallopeptidase domain 8 antibody Adam8 antibody ADAM8 protein antibody ADAM8_HUMAN antibody CD 156 antibody CD156 antibody CD156a antibody CD156a antigen antibody Cell surface antigen MS2 antibody Disintegrin and metalloproteinase domain-containing protein 8 antibody Human leukocyte differentiation antigen antibody Macrophage cysteine rich glycoprotein antibody MGC134985 antibody MS 2 antibody MS2 antibody |
| Molecular Weight(MW) | 100kDa |
| Cellular Localization | Membrane; Single-pass type I membrane protein. |
WB
Positive WB detected in: THP-1 whole cell lysate(20µg), K562 whole cell lysate(20µg), JK whole cell lysate(20µg), All lanes: ADAM8 antibody at 1:1000, Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Exposure time: 300s.
ICC/IF
Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:1000-1:5000 IHC:1:25-1:100 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
