WB
Western blot analysis of various lysates using Phospho-PKC (pan) (gamma)-T514 Rabbit pAb at 1:1000 dilution. RAW 264.7 cells were treated with PMA (200 nM) for 30 minutes .C6 cells were treated with PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.| Product Name | Phospho-PKC (pan) (gamma)-T514 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Summary | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2008] |
|---|---|
| Alternative Names | PRKCA/PRKCB/PRKCD/PRKCE/PRKCG/PRKCH/PRKCQ Phospho-PKC (pan) (gamma)-T514 |
| Molecular Weight(MW) | 28kDa |
| Cellular Localization | cytoplasm, cytosol, endoplasmic reticulum, extracellular exosome, mitochondrial membrane, mitochondrion, nucleoplasm, perinuclear region of cytoplasm, plasma membrane. |
WB
Western blot analysis of various lysates using Phospho-PKC (pan) (gamma)-T514 Rabbit pAb at 1:1000 dilution. RAW 264.7 cells were treated with PMA (200 nM) for 30 minutes .C6 cells were treated with PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.| Application Notes | WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
