WB
Western blot analysis of Osteopontin/SPP1 using anti-Osteopontin/SPP1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse small intestine tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates, Lane 7: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Osteopontin/SPP1 antigen affinity purified monoclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of SPP1 using anti-SPP1 antibody. SPP1 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-SPP1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
Immunofluorescent analysis using the Antibody.| Product Name | Rabbit monoclonal antibody to Osteopontin/SPP1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human Osteopontin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPP1 |
|---|---|
| Gene Synonyms | OPN BNSP BSPI ETA-1 |
| Gene Full Name | secreted phosphoprotein 1 |
| Gene Summary | The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| Alternative Names | BNSP Bone sialoprotein 1 BSPI ETA 1 Nephropontin OPN Osteopontin secreted phosphoprotein 1 SPP 1 SPP1 Urinary stone protein Uropontin |
| Molecular Weight(MW) | 35 kDa |
| Cellular Localization | Extracellular region or secreted. |
WB
Western blot analysis of Osteopontin/SPP1 using anti-Osteopontin/SPP1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse small intestine tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates, Lane 7: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Osteopontin/SPP1 antigen affinity purified monoclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of SPP1 using anti-SPP1 antibody. SPP1 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-SPP1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
Immunofluorescent analysis using the Antibody.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
