WB
Various lysates were subjected to SDS PAGE followed by western blot with FTSJD2 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using FTSJD2 antibody at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using FTSJD2 antibody at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using FTSJD2 antibody at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).