WB
Sample loading: Approximately 2 μ g of total protein from rapeseed and rice leaves, electrophoresis: 12.5% SDS-PAGE, membrane transfer: NC (nitrocellulose) membrane, voltage 10v overnight, blocking: 5% skim milk powder, incubation at room temperature for 1 hour, primary antibody: 1:5000 dilution, incubation at room temperature for 1 hour.| Product Name | Anti-HCF136/ Photosystem II stability/assembly factor HCF136 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT5G23120, O82660 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:5000 |
|
| Species Reactivity | ArabidopsisRice |
| Purification | Affinity purified |
| Gene Symbol | HCF136 |
|---|---|
| Gene Synonyms | HIGH CHLOROPHYLL FLUORESCENCE 136 MYJ24.11 MYJ24_11 |
| Gene Full Name | photosystem II stability/assembly factor, chloroplast (HCF136) |
| Molecular Weight(MW) | 44 / 35.6 kDa |
| Function | HCF136 encodes a stability and/or assembly factor of photosystem II. It is essential for photosystem II (PSII) biogenesis, and required for assembly of an early intermediate in PSII assembly that includes D2 (psbD) and cytochrome b559. |
WB
Sample loading: Approximately 2 μ g of total protein from rapeseed and rice leaves, electrophoresis: 12.5% SDS-PAGE, membrane transfer: NC (nitrocellulose) membrane, voltage 10v overnight, blocking: 5% skim milk powder, incubation at room temperature for 1 hour, primary antibody: 1:5000 dilution, incubation at room temperature for 1 hour.| Application Notes | WB:1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 50% glycerol，-20 °C |
