WB
Sample loading: 1ug Arabidopsis/rice leaf total protein, electrophoresis: 12.5% SDS-PAGE, membrane transfer: NC (nitrocellulose) membrane, voltage 10 V overnight, blocking: 5% skim milk powder, incubation at room temperature for 1 hour, primary antibody: 1:5000 dilution, incubation at room temperature for 1 hour.