WB
Various lysates were subjected to SDS PAGE followed by western blot with CKAP2L antibody at dilution of 1:2500 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer slide using CKAP2L antibody at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IP
IP result of anti-CKAP2L (IP: 4ug; Detection: 1:2000) with Jurkat cells lysate 1560 ug.| Product Name | CKAP2L Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human CKAP2L protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:1000-1:5000 IHC:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | Human |
| Concentration | 0.4mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CKAP2L |
|---|---|
| Gene Full Name | cytoskeleton associated protein 2 like |
| Gene Summary | The protein encoded by this gene is thought to be a mitotic spindle protein important to neural stem or progenitor cells. Mutations in this gene have been associated with spindle organization defects, including mitotic spindle defects, lagging chromosomes, and chromatin bridges. There is evidence that mutations in this gene are associated with Filippi syndrome, characterized by growth defects, microcephaly, intellectual disability, facial feature defects, and syndactyly. There is a pseudogene of this gene on chromosome 20. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015] |
| Alternative Names | Cytoskeleton-associated protein 2-like Radial fiber and mitotic spindle protein Radmis |
| Molecular Weight(MW) | 84kDa |
| Cellular Localization | Cytoplasm,Cytoskeleton. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with CKAP2L antibody at dilution of 1:2500 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer slide using CKAP2L antibody at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP
IP result of anti-CKAP2L (IP: 4ug; Detection: 1:2000) with Jurkat cells lysate 1560 ug.| Application Notes | WB:1:1000-1:5000 IHC:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
