WB
Western blot analysis of ERO1L on different lysates with Rabbit anti-ERO1L antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane), Lane 2: HepG2 cell lysate (20 µg/Lane), Lane 3: Jurkat cell lysate (20 µg/Lane), Lane 4: NIH/3T3 cell lysate (20 µg/Lane), Lane 5: L6 cell lysate (20 µg/Lane), Lane 6: Mouse placenta tissue lysate (40 µg/Lane), Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-ERO1L antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | ERO1L Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human ERO1L aa 1-468. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000 IHC:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ERO1A |
|---|---|
| Gene Synonyms | ERO1L ERO1-L ERO1LA Ero1alpha ERO1-alpha ERO1-L-alpha |
| Gene Full Name | endoplasmic reticulum oxidoreductase 1 alpha |
| Gene Summary | Enables oxidoreductase activity. Involved in cell redox homeostasis and chaperone cofactor-dependent protein refolding. Located in Golgi lumen; endoplasmic reticulum; and extracellular space. [provided by Alliance of Genome Resources, Apr 2025] |
| Alternative Names | Endoplasmic oxidoreductin 1 like protein antibody Endoplasmic oxidoreductin-1-like protein antibody Endoplasmic reticulum oxidoreductase 1 alpha antibody Endoplasmic reticulum oxidoreductin 1-like antibody ERO1 alpha antibody ERO1 L antibody ERO1 Lalpha antibody ERO1 like protein alpha antibody ERO1-alpha antibody ERO1-L antibody Endoplasmic oxidoreductin 1 like protein antibody Endoplasmic oxidoreductin-1-like protein antibody Endoplasmic reticulum oxidoreductase 1 alpha antibody Endoplasmic reticulum oxidoreductin 1-like antibody ERO1 alpha antibody ERO1 L antibody ERO1 Lalpha antibody ERO1 like protein alpha antibody ERO1-alpha antibody ERO1-L antibody ERO1-L-alpha antibody ERO1-like (S. cerevisiae) antibody ERO1-like alpha antibody ERO1-like protein alpha antibody ERO1-like S. cerevisiae homolog of alpha antibody ERO1A antibody ERO1A_HUMAN antibody ERO1L antibody ERO1LA antibody Oxidoreductin 1 Lalpha antibody Oxidoreductin-1-L-alpha antibody PRO865 antibody UNQ434 antibody |
| Molecular Weight(MW) | 54kDa(Observed band size: 60 kDa) |
| Cellular Localization | Endoplasmic reticulum membrane, membrane protein, Golgi apparatus lumen, Secreted, Cell projection, dendrite. |
WB
Western blot analysis of ERO1L on different lysates with Rabbit anti-ERO1L antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane), Lane 2: HepG2 cell lysate (20 µg/Lane), Lane 3: Jurkat cell lysate (20 µg/Lane), Lane 4: NIH/3T3 cell lysate (20 µg/Lane), Lane 5: L6 cell lysate (20 µg/Lane), Lane 6: Mouse placenta tissue lysate (40 µg/Lane), Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-ERO1L antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000 IHC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
