WB
Direct Western blot analysis of MCT1 expression in whole cell lysate of A-673 (A), SJRH30 (B), HEL 92.1.7 (C), BT-20 (D), and Hep G2 (E)ICC/IF
Direct immunofluorescence staining of SW480 cells fixed in formalin showed membrane localization.IHC
Immunoperoxidase staining of human liver tissue fixed in formalin and embedded in paraffin reveals staining of liver cell membranes.| Product Name | Mouse Monoclonal antibody to MCT1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | raised against amino acids 191-260 mapping within a cytoplasmic domain of MCT1 of human origin. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:100-1:1000 ICC/IF:1:50-1:500 IHC:1:50-1:500 |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC16A1 |
|---|---|
| Gene Synonyms | MCT HHF7 MCT1 MCT1D |
| Gene Full Name | solute carrier family 16 member 1 |
| Gene Summary | The protein encoded by this gene is a proton-linked monocarboxylate transporter that catalyzes the movement of many monocarboxylates, such as lactate and pyruvate, across the plasma membrane. Mutations in this gene are associated with erythrocyte lactate transporter defect. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2009] |
| Molecular Weight(MW) | 40-48 kDa |
| Cellular Localization | Membrane. |
WB
Direct Western blot analysis of MCT1 expression in whole cell lysate of A-673 (A), SJRH30 (B), HEL 92.1.7 (C), BT-20 (D), and Hep G2 (E)
ICC/IF
Direct immunofluorescence staining of SW480 cells fixed in formalin showed membrane localization.
IHC
Immunoperoxidase staining of human liver tissue fixed in formalin and embedded in paraffin reveals staining of liver cell membranes.| Application Notes | WB:1:100-1:1000 ICC/IF:1:50-1:500 IHC:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
