WB
Western blot analysis of VGLUT2 on different lysates with Rabbit anti-VGLUT2 antibody at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate (20 µg/Lane), Lane 2: Mouse lung tissue lysate (negative) (20 µg/Lane), Lane 3: Rat brain tissue lysate (20 µg/Lane), Lane 4: Rat lung tissue lysate (negative) (20 µg/Lane), Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-VGLUT2 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-VGLUT2 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-VGLUT2 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-VGLUT2 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-VGLUT2 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-VGLUT2 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-F
Species: Mouse, Site: Cerebellum, Sample: Frozen section, Antibody concentration: 1: 500, Antigen retrieval: Not required.IF-F
Species: Rat, Site: Cerebellum, Sample: Frozen section, Antibody concentration: 1: 500, Antigen retrieval: Not required.IF-P
Species: Mouse, Site: Cerebellum, Sample: Paraffin-embedded section, Antibody concentration: 1/500 (VGLUT2, Rabbit, red); 1/500 (VGLUT1, Rat, green)| Product Name | VGLUT2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-FIF-PIHCWB |
| WB:1:2000 IHC:1:2000 IF-F:1:500 IF-P:1:500 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Slc17a6 |
|---|---|
| Gene Synonyms | DNPI VGLUT2 2900073D12Rik |
| Gene Full Name | solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6 |
| Gene Summary | Enables L-glutamate uniporter activity; potassium:proton antiporter activity; and sodium:phosphate symporter activity. Involved in several processes, including L-glutamate transmembrane transport; hyaloid vascular plexus regression; and sodium-dependent phosphate transport. Acts upstream of or within neurotransmitter uptake. Located in synaptic vesicle. Is expressed in several structures, including cranial ganglion; nervous system; sensory organ; tail mesenchyme; and testis. Orthologous to human SLC17A6 (solute carrier family 17 member 6). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | Differentiation associated BNPI antibody Differentiation associated Na dependent inorganic phosphate cotransporter antibody Differentiation associated Na(+) dependent inorganic phosphate cotransporter antibody Differentiation-associated BNPI antibody Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter antibody DNPI antibody SLC17A6 antibody Sodium dependent inorganic phosphate cotransporter antibody Solute carrier family 17 (Sodium dependent inorganic phosphate cotransporter) member 6 antibody Solute carrier family 17 member 6 antibody Differentiation associated BNPI antibody Differentiation associated Na dependent inorganic phosphate cotransporter antibody Differentiation associated Na(+) dependent inorganic phosphate cotransporter antibody Differentiation-associated BNPI antibody Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter antibody DNPI antibody SLC17A6 antibody Sodium dependent inorganic phosphate cotransporter antibody Solute carrier family 17 (Sodium dependent inorganic phosphate cotransporter) member 6 antibody Solute carrier family 17 member 6 antibody Vesicular glutamate transporter 2 antibody Vesicular glutamate transporter type 2 antibody VGLU2_HUMAN antibody VGLUT 2 antibody VGluT2 antibody |
| Molecular Weight(MW) | 65kDa |
| Cellular Localization | Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Synapse, synaptosome, Cell membrane. |
WB
Western blot analysis of VGLUT2 on different lysates with Rabbit anti-VGLUT2 antibody at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate (20 µg/Lane), Lane 2: Mouse lung tissue lysate (negative) (20 µg/Lane), Lane 3: Rat brain tissue lysate (20 µg/Lane), Lane 4: Rat lung tissue lysate (negative) (20 µg/Lane), Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-VGLUT2 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-VGLUT2 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-VGLUT2 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-VGLUT2 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-VGLUT2 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-VGLUT2 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-F
Species: Mouse, Site: Cerebellum, Sample: Frozen section, Antibody concentration: 1: 500, Antigen retrieval: Not required.
IF-F
Species: Rat, Site: Cerebellum, Sample: Frozen section, Antibody concentration: 1: 500, Antigen retrieval: Not required.
IF-P
Species: Mouse, Site: Cerebellum, Sample: Paraffin-embedded section, Antibody concentration: 1/500 (VGLUT2, Rabbit, red); 1/500 (VGLUT1, Rat, green)| Application Notes | WB:1:2000 IHC:1:2000 IF-F:1:500 IF-P:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
