WB
Western blot analysis of VGLUT2 on different lysates with Mouse anti-VGLUT2 antibody at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate, Lane 2: Mouse lung tissue lysate (negative), Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-VGLUT2 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-VGLUT2 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-F
Species: Mouse, Site: hippocampus, Sample: Frozen section, Antibody concentration: 1/500 (VGLUT2, Mouse, green); 1/500 (VGLUT1, Guinea pig, red)IF-F
Species: Rat, Site: hippocampus, Sample: Frozen section, Antibody concentration: 1/500 (VGLUT2, Mouse, green);1/500 (VGLUT1,Guinea pig, red).| Product Name | VGLUT2 Recombinant Antibody - Mouse IgG1 (Chimeric) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Tested Applications | IF-FIHCWB |
| WB:1:5000 IHC:1:2000 IF-F:1:500 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Slc17a6 |
|---|---|
| Gene Synonyms | DNPI VGLUT2 2900073D12Rik |
| Gene Full Name | solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6 |
| Gene Summary | Enables L-glutamate uniporter activity; potassium:proton antiporter activity; and sodium:phosphate symporter activity. Involved in several processes, including L-glutamate transmembrane transport; hyaloid vascular plexus regression; and sodium-dependent phosphate transport. Acts upstream of or within neurotransmitter uptake. Located in synaptic vesicle. Is expressed in several structures, including cranial ganglion; nervous system; sensory organ; tail mesenchyme; and testis. Orthologous to human SLC17A6 (solute carrier family 17 member 6). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | Differentiation associated BNPI antibody Differentiation associated Na dependent inorganic phosphate cotransporter antibody Differentiation associated Na(+) dependent inorganic phosphate cotransporter antibody Differentiation-associated BNPI antibody Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter antibody DNPI antibody SLC17A6 antibody Sodium dependent inorganic phosphate cotransporter antibody Solute carrier family 17 (Sodium dependent inorganic phosphate cotransporter) member 6 antibody Solute carrier family 17 member 6 antibody Vesicular glutamate transporter 2 antibody Vesicular glutamate transporter type 2 antibody VGLU2_HUMAN antibody VGLUT 2 antibody VGluT2 antibody |
| Molecular Weight(MW) | 65 kDa |
| Cellular Localization | Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Synapse, synaptosome, Cell membrane. |
WB
Western blot analysis of VGLUT2 on different lysates with Mouse anti-VGLUT2 antibody at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate, Lane 2: Mouse lung tissue lysate (negative), Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-VGLUT2 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-VGLUT2 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-F
Species: Mouse, Site: hippocampus, Sample: Frozen section, Antibody concentration: 1/500 (VGLUT2, Mouse, green); 1/500 (VGLUT1, Guinea pig, red)
IF-F
Species: Rat, Site: hippocampus, Sample: Frozen section, Antibody concentration: 1/500 (VGLUT2, Mouse, green);1/500 (VGLUT1,Guinea pig, red).| Application Notes | WB:1:5000 IHC:1:2000 IF-F:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
