WB
Western blot analysis was performed on the expression of Podocalyxin like 1 in ECV304 (A), H9 (B), Raji (C), and MIA PaCa-2 (D) whole cell lysates.IHC
Immunoperoxidase staining of human cortical tissue fixed in formalin and embedded in paraffin reveals cytoplasmic staining of neuronal and endothelial cells.IHC
Immunoperoxidase staining of human fallopian tube tissue fixed in formalin and embedded in paraffin reveals cytoplasmic staining of the apical membrane and glandular cells.ICC/IF
Immunofluorescence staining of methanol fixed HeLa cells revealed nuclear and cytoplasmic localization.| Product Name | Mouse Monoclonal antibody to Podocalyxin-like 1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | raised against human recombinant podocalyxin-like protein. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:100-1:1000 IHC:1:50-1:500 ICC/IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PODXL |
|---|---|
| Gene Synonyms | PC PDX PCLP Gp200 gp135 PCLP-1 PODXL1 |
| Gene Full Name | podocalyxin like |
| Gene Summary | This gene encodes an actin sequestering protein which plays a role in regulation of actin polymerization. The protein is also involved in cell proliferation, migration, and differentiation. This gene escapes X inactivation and has a homolog on chromosome Y. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 165 kDa |
| Cellular Localization | Apical cell membrane,Cell projection, microvillus. |
WB
Western blot analysis was performed on the expression of Podocalyxin like 1 in ECV304 (A), H9 (B), Raji (C), and MIA PaCa-2 (D) whole cell lysates.
IHC
Immunoperoxidase staining of human cortical tissue fixed in formalin and embedded in paraffin reveals cytoplasmic staining of neuronal and endothelial cells.
IHC
Immunoperoxidase staining of human fallopian tube tissue fixed in formalin and embedded in paraffin reveals cytoplasmic staining of the apical membrane and glandular cells.
ICC/IF
Immunofluorescence staining of methanol fixed HeLa cells revealed nuclear and cytoplasmic localization.| Application Notes | WB:1:100-1:1000 IHC:1:50-1:500 ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
