WB
Western blot analysis of ALK-1 on different lysates with Rabbit anti-ALK-1 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane), Lane 2: Human lung tissue lysate (40 µg/Lane), Lane 3: Mouse lung tissue lysate (40 µg/Lane), Lane 4: Rat lung tissue lysate (40 µg/Lane), Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-ALK-1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | ALK-1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within human ALK-1 aa 454-503 / 503. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000 IHC:1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ACVRL1 |
|---|---|
| Gene Synonyms | HHT ALK1 HHT2 ORW2 SKR3 ALK-1 TSR-I ACVRLK1 |
| Gene Full Name | activin A receptor like type 1 |
| Gene Summary | This gene encodes a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. The encoded protein, sometimes termed ALK1, shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in this gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2. [provided by RefSeq, Jul 2008] |
| Alternative Names | Activin A receptor antibody Activin A receptor type II like 1 antibody Activin A receptor type II like kinase 1 antibody Activin receptor like kinase 1 antibody Activin receptor-like kinase 1 antibody ACVL1_HUMAN antibody ACVRL1 antibody ACVRLK1 antibody ALK-1 antibody ALK1 antibody Activin A receptor antibody Activin A receptor type II like 1 antibody Activin A receptor type II like kinase 1 antibody Activin receptor like kinase 1 antibody Activin receptor-like kinase 1 antibody ACVL1_HUMAN antibody ACVRL1 antibody ACVRLK1 antibody ALK-1 antibody ALK1 antibody HHT antibody HHT2 antibody ORW2 antibody Osler Rendu Weber syndrome 2 antibody Serine/threonine protein kinase receptor R3 antibody Serine/threonine-protein kinase receptor R3 antibody SKR3 antibody TGF B superfamily receptor type I antibody TGF-B superfamily receptor type I antibody TSR-I antibody TSR1 antibody |
| Molecular Weight(MW) | 56kDa(Observed band size: 60kDa) |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of ALK-1 on different lysates with Rabbit anti-ALK-1 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane), Lane 2: Human lung tissue lysate (40 µg/Lane), Lane 3: Mouse lung tissue lysate (40 µg/Lane), Lane 4: Rat lung tissue lysate (40 µg/Lane), Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-ALK-1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000 IHC:1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
