WB
mouse liver tissue were subjected to SDS PAGE followed by western blot with FTCD antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.WB
rat liver tissue were subjected to SDS PAGE followed by western blot with FTCD antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using FTCD antibody at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using FTCD antibody at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF-P
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FTCD antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L).| Product Name | FTCD Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human FTCD protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | IF-PIHCWB |
| WB:1:2000-1:10000 IHC:1:150-1:600 IF-P:1:200-1:800 |
|
| Species Reactivity | HumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FTCD |
|---|---|
| Gene Synonyms | LCHC1 |
| Gene Full Name | formimidoyltransferase cyclodeaminase |
| Gene Summary | The protein encoded by this gene is a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Mutations in this gene are associated with glutamate formiminotransferase deficiency. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Dec 2009] |
| Alternative Names | Formimidoyltetrahydrofolate cyclodeaminase Formimidoyltransferase-cyclodeaminase Formiminotransferase-cyclodeaminase Glutamate formimidoyltransferase Glutamate formiminotransferase |
| Molecular Weight(MW) | 53-62 kDa |
| Cellular Localization | Cytoplasm,Cytoskeleton,Golgi apparatus. |
WB
mouse liver tissue were subjected to SDS PAGE followed by western blot with FTCD antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB
rat liver tissue were subjected to SDS PAGE followed by western blot with FTCD antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using FTCD antibody at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using FTCD antibody at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FTCD antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L).| Application Notes | WB:1:2000-1:10000 IHC:1:150-1:600 IF-P:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
