WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody.WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-TGF β1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.IHC
Mouse spleen was stained with anti-TGF β1 rabbit antibody.IHC
Rat spleen was stained with anti-TGF β1 rabbit antibody.ICC/IF
Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4℃ overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, DAPI(blue) 10min.IF-P
Rat jejunum was stained with anti-TGF β1 Rabbit antibody.| Product Name | TGFβ1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCWB |
| WB:1:1000-1:5000 IHC:1:2000-1:5000 ICC/IF:1:200-1:1000 IF-P:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TGFB1 |
|---|---|
| Gene Synonyms | CED LAP DPD1 TGFB CAEND1 IBDIMDE TGFbeta TGF-beta1 |
| Gene Full Name | transforming growth factor beta 1 |
| Gene Summary | This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGFB family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease. [provided by RefSeq, Aug 2016] |
| Alternative Names | Transforming growth factor beta-1 TGF-beta-1 [Cleaved into: Latency-associated peptide LAP ] |
| Molecular Weight(MW) | 44kDa,13kDa |
| Cellular Localization | ecreted. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody.
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-TGF β1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
IHC
Mouse spleen was stained with anti-TGF β1 rabbit antibody.
IHC
Rat spleen was stained with anti-TGF β1 rabbit antibody.
ICC/IF
Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4℃ overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, DAPI(blue) 10min.
IF-P
Rat jejunum was stained with anti-TGF β1 Rabbit antibody.| Application Notes | WB:1:1000-1:5000 IHC:1:2000-1:5000 ICC/IF:1:200-1:1000 IF-P:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
