WB
Western blot analysis of FOXL2 on different lysates with Rabbit anti-FOXL2 antibody at 1/5,000 dilution. Lane 1: SiHa cell lysate (20 µg/Lane), Lane 2: THP-1 cell lysate (20 µg/Lane), Lane 3: K-562 cell lysate (20 µg/Lane), Lane 4: Mouse ovary tissue lysate (30 µg/Lane), Lane 5: Mouse testis tissue lysate (negative) (30 µg/Lane), Lane 6: Rat ovary tissue lysate (30 µg/Lane), Lane 7: Rat testis tissue lysate (negative) (30 µg/Lane), Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human ovary tissue with Rabbit anti-FOXL2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue with Rabbit anti-FOXL2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat ovary tissue with Rabbit anti-FOXL2 antibody at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human testis tissue (negative) with Rabbit anti-FOXL2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue (negative) with Rabbit anti-FOXL2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat testis tissue (negative) with Rabbit anti-FOXL2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IP
FOXL2 was immunoprecipitated from 0.2 mg K-562 cell lysate with Rabbit anti-FOXL2 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-FOXL2 antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: K-562 cell lysate (input), Lane 2: Rabbit anti-FOXL2 antibody IP in K-562 cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-FOXL2 antibody in K-562 cell lysate, Blocking/Dilution buffer: primary antibody dilution, Exposure time: 46 seconds.| Product Name | FOXL2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within mouse FOXL2 aa 320-375. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:5000 IHC:1:200 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | FOXL2 |
|---|---|
| Gene Synonyms | BPES PFRK POF3 BPES1 PINTO |
| Gene Full Name | forkhead box L2 |
| Gene Summary | This gene encodes a forkhead transcription factor. The protein contains a fork-head DNA-binding domain and may play a role in ovarian development and function. Expansion of a polyalanine repeat region and other mutations in this gene are a cause of blepharophimosis syndrome and premature ovarian failure 3. [provided by RefSeq, Jul 2016] |
| Alternative Names | Blepharophimosis antibody Blepharophimosis epicanthus inversus and ptosis 1 antibody Blepharophimosis epicanthus inversus and ptosis antibody BPES 1 antibody BPES antibody BPES1 antibody Epicanthus inversus and ptosis 1 antibody Forkhead box L2 antibody Forkhead box protein L2 antibody Forkhead transcription factor FOXL2 antibody Blepharophimosis antibody Blepharophimosis epicanthus inversus and ptosis 1 antibody Blepharophimosis epicanthus inversus and ptosis antibody BPES 1 antibody BPES antibody BPES1 antibody Epicanthus inversus and ptosis 1 antibody Forkhead box L2 antibody Forkhead box protein L2 antibody Forkhead transcription factor FOXL2 antibody FOX L2 antibody FOXL 2 antibody FOXL2 antibody FOXL2_HUMAN antibody PFRK antibody PINTO antibody PITUITARY FORKHEAD FACTOR antibody POF 3 antibody POF3 antibody |
| Molecular Weight(MW) | 39 kDa(Observed band size: 50 kDa) |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of FOXL2 on different lysates with Rabbit anti-FOXL2 antibody at 1/5,000 dilution. Lane 1: SiHa cell lysate (20 µg/Lane), Lane 2: THP-1 cell lysate (20 µg/Lane), Lane 3: K-562 cell lysate (20 µg/Lane), Lane 4: Mouse ovary tissue lysate (30 µg/Lane), Lane 5: Mouse testis tissue lysate (negative) (30 µg/Lane), Lane 6: Rat ovary tissue lysate (30 µg/Lane), Lane 7: Rat testis tissue lysate (negative) (30 µg/Lane), Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human ovary tissue with Rabbit anti-FOXL2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue with Rabbit anti-FOXL2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat ovary tissue with Rabbit anti-FOXL2 antibody at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human testis tissue (negative) with Rabbit anti-FOXL2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue (negative) with Rabbit anti-FOXL2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat testis tissue (negative) with Rabbit anti-FOXL2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IP
FOXL2 was immunoprecipitated from 0.2 mg K-562 cell lysate with Rabbit anti-FOXL2 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-FOXL2 antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: K-562 cell lysate (input), Lane 2: Rabbit anti-FOXL2 antibody IP in K-562 cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-FOXL2 antibody in K-562 cell lysate, Blocking/Dilution buffer: primary antibody dilution, Exposure time: 46 seconds.| Application Notes | WB:1:5000 IHC:1:200 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
