WB
Western blot analysis of FOXL2 using anti-FOXL2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FOXL2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of FOXL2 using anti-FOXL2 antibody. FOXL2 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-FOXL2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of FOXL2 using anti-FOXL2 antibody. FOXL2 was detected in a paraffin-embedded section of mouse ovary tissue. The tissue section was incubated with rabbit anti-FOXL2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of FOXL2 using anti-FOXL2 antibody. FOXL2 was detected in a paraffin-embedded section of rat ovary tissue. The tissue section was incubated with rabbit anti-FOXL2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.FC
Flow Cytometry analysis of THP-1 cells using anti-FOXL2 antibody. Overlay histogram showing THP-1 cells stained with Anti-FOXL2 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FOXL2 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to FOXL2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human FOXL2, identical to the related mouse sequences. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FOXL2 |
|---|---|
| Gene Synonyms | BPES PFRK POF3 BPES1 PINTO |
| Gene Full Name | forkhead box L2 |
| Gene Summary | This gene encodes a forkhead transcription factor. The protein contains a fork-head DNA-binding domain and may play a role in ovarian development and function. Expansion of a polyalanine repeat region and other mutations in this gene are a cause of blepharophimosis syndrome and premature ovarian failure 3. [provided by RefSeq, Jul 2016] |
| Alternative Names | BPES BPES1 forkhead box L2 Forkhead box protein L2 FOXL2 FOXL2-Specific PFRK PINTO POF3 |
| Molecular Weight(MW) | 39 kDa(Observed band size: 50 kDa) |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of FOXL2 using anti-FOXL2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FOXL2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of FOXL2 using anti-FOXL2 antibody. FOXL2 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-FOXL2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of FOXL2 using anti-FOXL2 antibody. FOXL2 was detected in a paraffin-embedded section of mouse ovary tissue. The tissue section was incubated with rabbit anti-FOXL2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of FOXL2 using anti-FOXL2 antibody. FOXL2 was detected in a paraffin-embedded section of rat ovary tissue. The tissue section was incubated with rabbit anti-FOXL2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
FC
Flow Cytometry analysis of THP-1 cells using anti-FOXL2 antibody. Overlay histogram showing THP-1 cells stained with Anti-FOXL2 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FOXL2 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
