FC
Blank control (blue line):Hela(blue). Primary Antibody (green line): Rabbit Anti-PDGF-B antibody Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice.Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | PDGF-B Rabbit pAb, BF647 conjugated |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from human PDGF-B : 155-210/241 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIF |
| IF:1:100-1:500 FC:1μg/Test |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PDGFB |
|---|---|
| Gene Synonyms | SIS SSV IBGC5 PDGF2 c-sis PDGF-2 |
| Gene Full Name | platelet derived growth factor subunit B |
| Gene Summary | This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit B, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit A. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with dermatofibrosarcoma protuberans, a rare skin tumor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015] |
| Alternative Names | PDGF beta PDGF-B Platelet-derived growth factor B-chain PDGF AB+BB Becaplermin c sis FLJ1285 Oncogene SIS PDGF 1 PDGF 2 PDGF A PDGF A chain PDGF B chain PDGF subunit A PDGF subunit B PDGF1 PDGF2 PDGFA PDGFB PDGFR1 Platelet derived growth factor 2 Platelet derived growth factor alpha Platelet derived growth factor alpha chain Platelet derived growth factor alpha polypeptide Platelet derived growth factor B chain Platelet derived growth factor beta Platelet derived growth factor beta polypeptide Platelet derived growth factor subunit A Platelet derived growth factor subunit B SIS SSV v sis platelet derived growth factor beta polypeptide. |
FC
Blank control (blue line):Hela(blue). Primary Antibody (green line): Rabbit Anti-PDGF-B antibody Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice.Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Application Notes | IF:1:100-1:500 FC:1μg/Test |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
