WB
Western blot analysis of various ysates using PDGFB Rabbit mAb at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60 s.IHC
Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using PDGFB Rabbit mAb at dlilution of 1:200 (40x lens) High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.IF
Confocal imaging of paraffin-embedded Mouse kidney tissue using PDGFB Rabbit mAb followed by a furtherincubation with Cy3 Goat Anti-Rabbit 1gG (H+L) (Red) DAPI was used for nuclear staining (Blue) High pressureantigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.| Product Name | PDGFB Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCWB |
| WB:1:500 - 1:2000 IF:1:200-1:800 IHC:1:200 - 1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PDGFB |
|---|---|
| Gene Synonyms | SIS SSV IBGC5 PDGF2 c-sis PDGF-2 |
| Gene Full Name | platelet derived growth factor subunit B |
| Gene Summary | This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit B, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit A. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with dermatofibrosarcoma protuberans, a rare skin tumor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015] |
| Alternative Names | SIS SSV IBGC5 PDGF2 c-sis PDGF-2 PDGFB |
| Molecular Weight(MW) | 27 kDa(Observed band size:35 kDa) |
| Cellular Localization | Secreted. |
WB
Western blot analysis of various ysates using PDGFB Rabbit mAb at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60 s.
IHC
Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using PDGFB Rabbit mAb at dlilution of 1:200 (40x lens) High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
IF
Confocal imaging of paraffin-embedded Mouse kidney tissue using PDGFB Rabbit mAb followed by a furtherincubation with Cy3 Goat Anti-Rabbit 1gG (H+L) (Red) DAPI was used for nuclear staining (Blue) High pressureantigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.| Application Notes | WB:1:500 - 1:2000 IF:1:200-1:800 IHC:1:200 - 1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
