WB
Western blot analysis of ASS1 using anti-ASS1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human MDA-MB-453 whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human HL-60 whole cell lysates, Lane 7: human U87 whole cell lysates, Lane 8: rat kidney tissue lysates, Lane 8: rat liver tissue lysates, Lane 10: mouse kidney tissue lysates, Lane 11: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ASS1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
ASS1 was detected in a paraffin-embedded section of human Pancreatic Cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ASS1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ASS1 was detected in an immunocytochemical section of HepG2 cells. The section was incubated with rabbit anti-ASS1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Overlay histogram showing THP-1 cells stained withanti-ASS1 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ASS1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to ASS1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human ASS1 recombinant protein (Position: S3-S365). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ASS1 |
|---|---|
| Gene Synonyms | ASS CTLN1 |
| Gene Full Name | argininosuccinate synthase 1 |
| Gene Summary | The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of this gene cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2012] |
| Alternative Names | Argininosuccinate synthase argininosuccinate synthetase 1 ASS ASS1 Citrulline aspartate ligase CTLN1 |
| Molecular Weight(MW) | 47 kDa |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of ASS1 using anti-ASS1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human MDA-MB-453 whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human HL-60 whole cell lysates, Lane 7: human U87 whole cell lysates, Lane 8: rat kidney tissue lysates, Lane 8: rat liver tissue lysates, Lane 10: mouse kidney tissue lysates, Lane 11: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ASS1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
ASS1 was detected in a paraffin-embedded section of human Pancreatic Cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ASS1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ASS1 was detected in an immunocytochemical section of HepG2 cells. The section was incubated with rabbit anti-ASS1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Overlay histogram showing THP-1 cells stained withanti-ASS1 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ASS1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
