WB
Western blot analysis of DAP-3 expression in SW-13 whole cell lysate.ICC/IF
Immunofluorescence staining of human endothelial cells reveals cytoplasmic localization.| Product Name | Mouse Monoclonal antibody to DAP-3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | amino acids 98-248 of DAP-3 of human origin |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| WB:1:100-1:1000 ICC/IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DAP3 |
|---|---|
| Gene Synonyms | mS29 DAP-3 S29mt MRPS29 PRLTS7 MRP-S29 bMRP-10 |
| Gene Full Name | death associated protein 3 |
| Gene Summary | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq, Dec 2010] |
| Molecular Weight(MW) | 46 kDa |
WB
Western blot analysis of DAP-3 expression in SW-13 whole cell lysate.
ICC/IF
Immunofluorescence staining of human endothelial cells reveals cytoplasmic localization.| Application Notes | WB:1:100-1:1000 ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 50 µg IgG1 in 500 µl of PBS with < 0.1% sodium azide, 0.1% gelatin, 20% glycerol and 0.04% stabilizer protein. |
