WB
Western blot analysis of nestin expression in SK-N-SH (A) and SH-SY5Y (B) whole cell lysate.IHC
Immunoperoxidase staining of human kidney tissue fixed in formalin and embedded in paraffin reveals cytoplasmic and membrane staining of glomerular cells.ICC/IF
Immunofluorescence staining of SW480 cells fixed in formalin reveals cytoplasmic and membrane localization.| Product Name | Mouse Monoclonal antibody to nestin |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | raised against a 150 amino acid epitope mapping near the C-terminus of human nestin. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:100-1:1000 IHC:1:50-1:500 ICC/IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NES |
|---|---|
| Gene Synonyms | Nbla00170 |
| Gene Full Name | nestin |
| Gene Summary | This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011] |
| Molecular Weight(MW) | 300 kDa |
WB
Western blot analysis of nestin expression in SK-N-SH (A) and SH-SY5Y (B) whole cell lysate.
IHC
Immunoperoxidase staining of human kidney tissue fixed in formalin and embedded in paraffin reveals cytoplasmic and membrane staining of glomerular cells.
ICC/IF
Immunofluorescence staining of SW480 cells fixed in formalin reveals cytoplasmic and membrane localization.| Application Notes | WB:1:100-1:1000 IHC:1:50-1:500 ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 50 µg IgG1 in 500 µl of PBS with < 0.1% sodium azide, 0.1% gelatin, 20% glycerol and 0.04% stabilizer protein. |
