WB
Western blot analysis of karyopherin β 1 expression in Y79 (A), NIH/3T3 (B), 3T3-L1 (C), C6 (D), and PC-12 (E) whole cell lysates.ICC/IF
Immunofluorescence staining of HeLa cells fixed with methanol reveals perinuclear localization.| Product Name | Mouse Monoclonal antibody to karyopherin β1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | between amino acids 851-876 at the C-terminus of karyopherin b1 of human origin. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| WB:1:100-1:1000 ICC/IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KPNB1 |
|---|---|
| Gene Synonyms | IMB1 IPO1 IPOB Impnb NTF97 |
| Gene Full Name | karyopherin subunit beta 1 |
| Gene Summary | Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2013] |
| Molecular Weight(MW) | 97 kDa |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
Western blot analysis of karyopherin β 1 expression in Y79 (A), NIH/3T3 (B), 3T3-L1 (C), C6 (D), and PC-12 (E) whole cell lysates.
ICC/IF
Immunofluorescence staining of HeLa cells fixed with methanol reveals perinuclear localization.| Application Notes | WB:1:100-1:1000 ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
