WB
Western blot analysis of SP110 on different lysates with Rabbit anti-SP110 antibody at 1/1,000 dilution. Lane 1: Jurkat cell lysate, Lane 2: HeLa cell lysate, Lane 3: HL-60 cell lysate, Lane 4: SW480 cell lysate, Lane 5: Daudi cell lysate, Lane 6: K-562 cell lysate, Lane 7: PC-3M cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-SP110 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | SP110 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human SP110 aa 301-550 / 689. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000 IHC:1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SP110 |
|---|---|
| Gene Synonyms | IPR1 VODI IFI41 IFI75 |
| Gene Full Name | SP110 nuclear body protein |
| Gene Summary | The nuclear body is a multiprotein complex that may have a role in the regulation of gene transcription. This gene is a member of the SP100/SP140 family of nuclear body proteins and encodes a leukocyte-specific nuclear body component. The protein can function as an activator of gene transcription and may serve as a nuclear hormone receptor coactivator. In addition, it has been suggested that the protein may play a role in ribosome biogenesis and in the induction of myeloid cell differentiation. Alternative splicing has been observed for this gene and three transcript variants, encoding distinct isoforms, have been identified. [provided by RefSeq, Jul 2008] |
| Alternative Names | FLJ22835 antibody IFI 41 antibody IFI 75 antibody IFI41 antibody IFI75 antibody Interferon induced protein 41 30kD antibody Interferon induced protein 41 antibody Interferon induced protein 41/75 antibody Interferon induced protein 75 52kD antibody Interferon induced protein 75 antibody FLJ22835 antibody IFI 41 antibody IFI 75 antibody IFI41 antibody IFI75 antibody Interferon induced protein 41 30kD antibody Interferon induced protein 41 antibody Interferon induced protein 41/75 antibody Interferon induced protein 75 52kD antibody Interferon induced protein 75 antibody Interferon-induced protein 41/75 antibody IPR 1 antibody IPR1 antibody Phosphoprotein 41 antibody Phosphoprotein 75 antibody SP 110 antibody SP110 antibody Sp110 nuclear body protein antibody SP110_HUMAN antibody Speckled 110 kDa antibody Transcriptional coactivator Sp110 antibody VODI antibody |
| Molecular Weight(MW) | 78 kDa(Observed band size:29-78 kDa) |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of SP110 on different lysates with Rabbit anti-SP110 antibody at 1/1,000 dilution. Lane 1: Jurkat cell lysate, Lane 2: HeLa cell lysate, Lane 3: HL-60 cell lysate, Lane 4: SW480 cell lysate, Lane 5: Daudi cell lysate, Lane 6: K-562 cell lysate, Lane 7: PC-3M cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-SP110 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000 IHC:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
