WB
Western blot analysis of extracts of normal 293T cells and 293T trans fected with NSP2 Protein, using SARS-CoV-2 NSP2 antibody at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 5s.