WB
Positive WB detected in: HepG2 whole cell lysate(30µg),Mouse Stomach tissue lysate(30µg),Mouse Small intestine tissue lysate(30µg)
All lanes: NMU antibody at 1:1000,
Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution,
Exposure time: 120s.
ICC/IF
Immunofluorescence staining of Hela cell with NMU Antibody at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).