WB
Positive WB detected in: SY5Y whole cell lysate, MCF7 whole cell lysate, 293 whole cell lysate, PC-3 whole cell lysate, All lanes: ABCA3 antibody at 1:1000, Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.ICC/IF
Immunofluorescence staining of MCF-7 cell with ABCA3 Antibody at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).ICC/IF
Immunofluorescence staining of SH-SY5Y cell with ABCA3 Antibody at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-ABCA3 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide of human ABCA3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:20-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ABCA3 |
|---|---|
| Gene Synonyms | ABC3 ABC-C SMDP3 LBM180 EST111653 |
| Gene Full Name | ATP binding cassette subfamily A member 3 |
| Gene Summary | The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. The full transporter encoded by this gene may be involved in development of resistance to xenobiotics and engulfment during programmed cell death. [provided by RefSeq, Jul 2008] |
| Alternative Names | ABC 3 antibody ABC C antibody ABC C transporter antibody ABC transporter 3 antibody ABC-C transporter antibody ABC3 antibody ABCA 3 antibody Abca3 antibody ABCA3 protein antibody ABCA3_HUMAN antibody ABCC antibody ATP binding cassette 3 antibody ATP binding cassette sub family A (ABC1) member 3 antibody ATP binding cassette sub family A member 3 antibody ATP binding cassette transporter 3 antibody ATP-binding cassette 3 antibody ATP-binding cassette sub-family A member 3 antibody ATP-binding cassette transporter 3 antibody CED7. C. elegans homolog of antibody EST111653 antibody LBM 180 antibody LBM180 antibody MGC72201 antibody P180 Lamellar Body Protein antibody SMDP3 antibody |
| Molecular Weight(MW) | 192 kDa |
| Cellular Localization | Endosome, multivesicular body membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane. Late endosome membrane. Lysosome membrane. |
WB
Positive WB detected in: SY5Y whole cell lysate, MCF7 whole cell lysate, 293 whole cell lysate, PC-3 whole cell lysate, All lanes: ABCA3 antibody at 1:1000, Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.
ICC/IF
Immunofluorescence staining of MCF-7 cell with ABCA3 Antibody at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
ICC/IF
Immunofluorescence staining of SH-SY5Y cell with ABCA3 Antibody at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:2000 ICC/IF:1:20-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
