WB
Western blot analysis of VEGFR3/FLT4 using anti-VEGFR3/FLT4 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VEGFR3/FLT4 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
VEGFR3/FLT4 was detected in a paraffin-embedded section of human prostatic cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR3/FLT4 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
VEGFR3/FLT4 was detected in a paraffin-embedded section of mouse liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR3/FLT4 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
VEGFR3/FLT4 was detected in a paraffin-embedded section of rat liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR3/FLT4 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
VEGFR3/FLT4 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-VEGFR3/FLT4 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Overlay histogram showing U2OS cells stained with anti-VEGFR3/FLT4 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VEGFR3/FLT4 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to VEGFR3/FLT4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human VEGF Receptor 3 recombinant protein (Position: Y25-N259). Human VEGF Receptor 3 shares 85.5% and 87.7% amino acid (aa) sequence identity with mouse and rat VEGF Receptor 3, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FLT4 |
|---|---|
| Gene Synonyms | PCL CHTD7 FLT-4 FLT41 LMPH1A LMPHM1 VEGFR3 VEGFR-3 |
| Gene Full Name | fms related receptor tyrosine kinase 4 |
| Gene Summary | This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. Mutations in this gene cause hereditary lymphedema type IA. [provided by RefSeq, Jul 2008] |
| Alternative Names | FLT 4 FLT4 FLT41 Fms like tyrosine kinase 4 fms related tyrosine kinase 4 LMPH1A PCL VEGFR 3 VEGFR3 |
| Molecular Weight(MW) | 153 kDa |
| Cellular Localization | Cell membrane,Cytoplasm,Nucleus,Secreted. |
WB
Western blot analysis of VEGFR3/FLT4 using anti-VEGFR3/FLT4 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VEGFR3/FLT4 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
VEGFR3/FLT4 was detected in a paraffin-embedded section of human prostatic cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR3/FLT4 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
VEGFR3/FLT4 was detected in a paraffin-embedded section of mouse liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR3/FLT4 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
VEGFR3/FLT4 was detected in a paraffin-embedded section of rat liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR3/FLT4 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
VEGFR3/FLT4 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-VEGFR3/FLT4 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Overlay histogram showing U2OS cells stained with anti-VEGFR3/FLT4 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VEGFR3/FLT4 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
