WB
Sample loading: Approximately 2 μ g total protein electrophoresis of Arabidopsis and rice leaves: 10% SDS-PAGE membrane transfer: NC (nitrocellulose) membrane, voltage 10v overnight blocking: 5% skim milk powder, room temperature incubation for 1 hour primary antibody: 1:5000 dilution, room temperature incubation for 1 hour.| Product Name | Anti-TUB3/ Tubulin beta-3 chain Rabbit Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant partprotein of Arabidopsis thaliana AT5G62700, Q9ASR0 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
|
| Species Reactivity | ArabidopsisRice |
| Purification | Affinity purified |
| Gene Synonyms | TUB3, Tubulinbeta-2/beta-3 chain |
|---|---|
| Molecular Weight(MW) | 50.7 kDa |
| Function | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. |
WB
Sample loading: Approximately 2 μ g total protein electrophoresis of Arabidopsis and rice leaves: 10% SDS-PAGE membrane transfer: NC (nitrocellulose) membrane, voltage 10v overnight blocking: 5% skim milk powder, room temperature incubation for 1 hour primary antibody: 1:5000 dilution, room temperature incubation for 1 hour.| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 50% glycerol，-20 °C |
