WB
Various lysates were subjected to SDS PAGE followed by western blot with KIF21A antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB
mouse brain tissue were subjected to SDS PAGE followed by western blot with KIF21A Antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using KIF21A antibody at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP
IP result of anti-KIF21A (IP:4ug; Detection: 1:500) with mouse brain tissue lysate 5600ug.