WB
Western blot analysis of SLC6A20 using anti-SLC6A20 antibod. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CCRF-CEM whole cell lysates, Lane 2: human COLO 320 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SLC6A20 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
SLC6A20 was detected in a paraffin-embedded section of human colon tissue. The tissue section was incubated with rabbit anti-SLC6A20 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
SLC6A20 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. The tissue section was incubated with rabbit anti-SLC6A20 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
SLC6A20 was detected in a paraffin-embedded section of mouse colon tissue. The tissue section was incubated with rabbit anti-SLC6A20 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.FC
Overlay histogram showing 293T cells stained with rabbit anti-SLC6A20 Antibody (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC6A20 Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Rabbit polyclonal antibody to SLC6A20 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human SLC6A20 recombinant protein (Position: S124-A555). Human SLC6A20 shares 91.9% and 90.3% amino acid (aa) sequence identity with mouse and rat SLC6A20, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC6A20 |
|---|---|
| Gene Synonyms | XT3 SIT1 IMINO Xtrp3 |
| Gene Full Name | solute carrier family 6 member 20 |
| Gene Summary | Transport of small hydrophilic substances across cell membranes is mediated by substrate-specific transporter proteins which have been classified into several families of related genes. The protein encoded by this gene belongs to the sodium:neurotransmitter symporter (SNF) family and functions as a proline transporter expressed in kidney and small intestine. Mutations in this gene are associated with Hyperglycinuria and Iminoglycinuria. [provided by RefSeq, Jul 2020] |
| Alternative Names | Sodium- and chloride-dependent transporter XTRP3 Sodium/imino-acid transporter 1 Solute carrier family 6 member 20 Transporter rB21A homolog SLC6A20 SIT1 XT3 XTRP3 |
| Molecular Weight(MW) | 66 kDa(Observed MW 70 kDa) |
| Cellular Localization | Apical cell membrane. |
WB
Western blot analysis of SLC6A20 using anti-SLC6A20 antibod. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CCRF-CEM whole cell lysates, Lane 2: human COLO 320 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SLC6A20 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
SLC6A20 was detected in a paraffin-embedded section of human colon tissue. The tissue section was incubated with rabbit anti-SLC6A20 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
SLC6A20 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. The tissue section was incubated with rabbit anti-SLC6A20 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
SLC6A20 was detected in a paraffin-embedded section of mouse colon tissue. The tissue section was incubated with rabbit anti-SLC6A20 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
FC
Overlay histogram showing 293T cells stained with rabbit anti-SLC6A20 Antibody (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC6A20 Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
