WB
Western blot analysis of CDK5RAP3 on different lysates with Rabbit anti-CDK5RAP3 antibody at 1/5,000 dilution. Lane 1: HeLa cell lysate, Lane 2: A431 cell lysate, Lane 3: MCF7 cell lysate, Lane 4: EL4 cell lysate, Lane 5: PC-12 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 39 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-CDK5RAP3 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat stomach tissue with Rabbit anti-CDK5RAP3 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of PC-12 cells labeling CDK5RAP3 with Rabbit anti-CDK5RAP3 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CDK5RAP3 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Product Name | CDK5RAP3 Rabbit Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human CDK5RAP3 aa 296-506 / 506. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:5000 IHC:1:200 ICC/IF:1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDK5RAP3 |
|---|---|
| Gene Synonyms | C53 IC53 LZAP HSF-27 MST016 PP1553 OK/SW-cl.114 |
| Gene Full Name | CDK5 regulatory subunit associated protein 3 |
| Gene Summary | This gene encodes a protein that has been reported to function in signaling pathways governing transcriptional regulation and cell cycle progression. It may play a role in tumorigenesis and metastasis. A pseudogene of this gene is located on the long arm of chromosome 20. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, May 2013] |
| Alternative Names | C53 antibody CDK5 activator binding protein C53 antibody CDK5 activator-binding protein C53 antibody CDK5 regulatory subunit associated protein 3 antibody CDK5 regulatory subunit-associated protein 3 antibody Cdk5rap3 antibody CK5P3_HUMAN antibody HSF 27 antibody HSF27 antibody IC53 antibody C53 antibody CDK5 activator binding protein C53 antibody CDK5 activator-binding protein C53 antibody CDK5 regulatory subunit associated protein 3 antibody CDK5 regulatory subunit-associated protein 3 antibody Cdk5rap3 antibody CK5P3_HUMAN antibody HSF 27 antibody HSF27 antibody IC53 antibody Ischemic heart CDK5 activator binding protein C53 antibody LXXLL/leucine zipper containing ARFbinding protein antibody LZAP antibody MST016 antibody OK/SW cl.114 antibody PP1553 antibody Protein HSF-27 antibody |
| Molecular Weight(MW) | 57 kDa(Observed band size: 60 kDa) |
| Cellular Localization | Cytoplasm, Cytoskeleton, Nucleus. |
WB
Western blot analysis of CDK5RAP3 on different lysates with Rabbit anti-CDK5RAP3 antibody at 1/5,000 dilution. Lane 1: HeLa cell lysate, Lane 2: A431 cell lysate, Lane 3: MCF7 cell lysate, Lane 4: EL4 cell lysate, Lane 5: PC-12 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 39 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-CDK5RAP3 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat stomach tissue with Rabbit anti-CDK5RAP3 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of PC-12 cells labeling CDK5RAP3 with Rabbit anti-CDK5RAP3 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CDK5RAP3 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:5000 IHC:1:200 ICC/IF:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
