WB
25 ug total protein per lane of various lysates (see on figure) probed with Nurr1 polyclonal antibody, unconjugated at 1:800 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.IHC
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nurr1 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit and DAB staining.IHC
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nurr1 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit and DAB staining.IHC
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nurr1 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit and DAB staining.| Product Name | Nurr1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from human Nurr1 : 301-400/598 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:200-1:1000 IHC:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NR4A2 |
|---|---|
| Gene Synonyms | NOT RNR1 HZF-3 IDLDP NURR1 TINUR |
| Gene Full Name | nuclear receptor subfamily 4 group A member 2 |
| Gene Summary | This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. The encoded protein may act as a transcription factor. Mutations in this gene have been associated with disorders related to dopaminergic dysfunction, including Parkinson disease, schizophernia, and manic depression. Misregulation of this gene may be associated with rheumatoid arthritis. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008] |
| Alternative Names | HZF-3 IDLDP NOT NURR1 RNR1 TINUR RNR-1 TINOR NR4A2_HUMAN NR4A2 Immediate-early response protein NOT Orphan nuclear receptor NURR1 Transcriptionally-inducible nuclear receptor NR4A2_MOUSE NUR-related factor 1 NR4A2_RAT Nuclear orphan receptor HZF-3 Regenerating liver nuclear receptor 1 (RNR-1) SL-322 nuclear receptor subfamily 4 group A member 2 nuclear receptor subfamily 4 group A member 2 |
| Molecular Weight(MW) | 67kDa |
| Cellular Localization | Nucleus. |
WB
25 ug total protein per lane of various lysates (see on figure) probed with Nurr1 polyclonal antibody, unconjugated at 1:800 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
IHC
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nurr1 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit and DAB staining.
IHC
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nurr1 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit and DAB staining.
IHC
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nurr1 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit and DAB staining.| Application Notes | WB:1:200-1:1000 IHC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
