Application
Western blot analysis in Hela cell line lysates (35ug/lane).Application
Confocal immunofluorescent analysis of NURR1 (NR4A2) Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with NURR1 (NR4A2) antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of anti-NURR1(NR4A2) Pab in mouse brain tissue lysate.| Product Name | NURR1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Nuclear receptor subfamily 4 group A member 2, Immediate-early response protein NOT, Orphan nuclear receptor NURR1, Transcriptionally-inducible nuclear receptor, NR4A2, NOT, NURR1, TINUR |
| Product description | Parkinson's disease (PD) is a multifactorial disease that appears to arise from the effects of both genetic and environmental influences. The known genetic factors include multiple genes that have been identified in related parkinsonian syndromes, as well as alpha-synuclein. Genes associated with either PD or Parkinson-related disorders include parkin, DJ-1, ubiquitin C-terminal hydrolase isozyme L1 (UCH-L1), nuclear receptor-related factor 1 (NURR1), and alpha-synuclein. Nurr1 is a transcription factor that is expressed in the embryonic ventral midbrain and is critical for the development of dopamine (DA) neurons. It belongs to the conserved family of nuclear receptors but lacks an identified ligand and is therefore referred to as an orphan receptor. RXR ligands can promote the survival of DA neurons via a process that depends on Nurr1-RXR heterodimers. In developing DA cells, Nurr1 is required for the expression of several genes important for DA synthesis and function. Nurr1 is also important for the maintenance of adult DA neurons.1) Perlmann T, et al. Cell Tissue Res. 318(1):45-52 (2004) Hsu HC,et al. Curr Drug Targets Inflamm Allergy. 3(4):413-23 (2004) Wallen-Mackenzie A, et al. Genes Dev. 17(24):3036-47 (2003) Ichinose,H., et al. Gene 230 (2), 233-239 (1999) Okabe,T., et al. J. Immunol. 154 (8), 3871-3879 (1995) Mages,H.W., et al. Mol. Endocrinol.(11), 1583-1591 (1994) |
| Immunogen | This NURR1 (NR4A2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human NURR1 (NR4A2). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | NR4A2 |
|---|---|
| Alternative Names | Nuclear receptor subfamily 4 group A member 2 Immediate-early response protein NOT Orphan nuclear receptor NURR1 Transcriptionally-inducible nuclear receptor NR4A2 NOT NURR1 TINUR |
| Molecular Weight(MW) | 67 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Western blot analysis in Hela cell line lysates (35ug/lane).
Application
Confocal immunofluorescent analysis of NURR1 (NR4A2) Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with NURR1 (NR4A2) antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of anti-NURR1(NR4A2) Pab in mouse brain tissue lysate.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
