WB
Western blot analysis of Strep-Tag II on different lysates with Rabbit anti-Strep-Tag II antibody at 1/10,000 dilution. Lane 1: 293T transfected with Strep-Tag II-tagged Histone H3.1 (C-terminal) cell lysate, Lane 2: 293T transfected with Strep-Tag II-tagged ACAT2 (N-terminal) cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of HeLa cells labeling Strep-Tag II with Rabbit anti-Strep-Tag II antibody at 1/2,500 dilution. HeLa cells, transfected with Strep-Tag II-tagged Histone H3.1 (C-terminal) or ACAT2 (N-terminal) expression vector, respectively, were fixed in 4% paraformaldehyde for 10 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Strep-Tag II antibody at 1/2,500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.IP
Strep-Tag II was immunoprecipitated from 0.2 mg 293T transfected with Strep-Tag II-tagged Histone H3.1 (C-terminal) cell lysate with Rabbit anti-Strep-Tag II antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-Strep-Tag II antibody at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: 293T transfected with Strep-Tag II-tagged Histone H3.1 (C-terminal) cell lysate (input), Lane 2: Rabbit anti-Strep-Tag II antibody IP in 293T transfected with Strep-Tag II-tagged Histone H3.1 (C-terminal) cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-Strep-Tag II antibody in 293T transfected with Strep-Tag II-tagged Histone H3.1 (C-terminal) cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 2 seconds.IP
Strep-Tag II was immunoprecipitated from 0.2 mg 293T transfected with Strep-Tag II-tagged ACAT2 (N-terminal) cell lysate with Rabbit anti-Strep-Tag II antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-Strep-Tag II antibody at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: 293T transfected with Strep-Tag II-tagged ACAT2 (N-terminal) cell lysate (input), Lane 2:Rabbit anti-Strep-Tag II antibody IP in 293T transfected with Strep-Tag II-tagged ACAT2 (N-terminal) cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-Strep-Tag II antibody in 293T transfected with Strep-Tag II-tagged ACAT2 (N-terminal) cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 2 seconds.| Product Name | Strep-Tag II Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide corresponding to Strep-tag II. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| WB:1:10000 ICC/IF:1:2500 IP:1-2μg/sample |
|
| Species Reactivity | All |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | anti-Strep-Tag II antibody Strep-Tag II antibody Strep-Tag II antibody antibody Strep-Tag IIantiboy antibody |
|---|---|
| Function | The Strep-tag® system is a method which allows the purification and detection of proteins by affinity chromatography. The Strep-tag II is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin®, a specifically engineered streptavidin, and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins. |
WB
Western blot analysis of Strep-Tag II on different lysates with Rabbit anti-Strep-Tag II antibody at 1/10,000 dilution. Lane 1: 293T transfected with Strep-Tag II-tagged Histone H3.1 (C-terminal) cell lysate, Lane 2: 293T transfected with Strep-Tag II-tagged ACAT2 (N-terminal) cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of HeLa cells labeling Strep-Tag II with Rabbit anti-Strep-Tag II antibody at 1/2,500 dilution. HeLa cells, transfected with Strep-Tag II-tagged Histone H3.1 (C-terminal) or ACAT2 (N-terminal) expression vector, respectively, were fixed in 4% paraformaldehyde for 10 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Strep-Tag II antibody at 1/2,500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
IP
Strep-Tag II was immunoprecipitated from 0.2 mg 293T transfected with Strep-Tag II-tagged Histone H3.1 (C-terminal) cell lysate with Rabbit anti-Strep-Tag II antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-Strep-Tag II antibody at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: 293T transfected with Strep-Tag II-tagged Histone H3.1 (C-terminal) cell lysate (input), Lane 2: Rabbit anti-Strep-Tag II antibody IP in 293T transfected with Strep-Tag II-tagged Histone H3.1 (C-terminal) cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-Strep-Tag II antibody in 293T transfected with Strep-Tag II-tagged Histone H3.1 (C-terminal) cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 2 seconds.
IP
Strep-Tag II was immunoprecipitated from 0.2 mg 293T transfected with Strep-Tag II-tagged ACAT2 (N-terminal) cell lysate with Rabbit anti-Strep-Tag II antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-Strep-Tag II antibody at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: 293T transfected with Strep-Tag II-tagged ACAT2 (N-terminal) cell lysate (input), Lane 2:Rabbit anti-Strep-Tag II antibody IP in 293T transfected with Strep-Tag II-tagged ACAT2 (N-terminal) cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-Strep-Tag II antibody in 293T transfected with Strep-Tag II-tagged ACAT2 (N-terminal) cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 2 seconds.| Application Notes | WB:1:10000 ICC/IF:1:2500 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
