IHC
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HMG2L1 Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit instructionsand DAB staining.IHC
Paraformaldehyde-fixed, paraffin embedded (Rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HMG2L1 Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit instructionsand DAB staining.| Product Name | HMG2L1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from human HMG2L1 : 1-100/601 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC |
| IHC:1:100-1:500 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | HMGXB4 |
|---|---|
| Gene Synonyms | P53N HMG2L1 HMGBCG THC211630 |
| Gene Full Name | HMG-box containing 4 |
| Gene Summary | High mobility group (HMG) proteins are nonhistone chromosomal proteins. See HMG2 (MIM 163906) for additional information on HMG proteins.[supplied by OMIM, Nov 2010] |
| Alternative Names | HMG2L1 HMGBCG P53N THC211630 HMGX4_HUMAN HMGXB4 HMG box-containing protein 4 High mobility group protein 2-like 1 Protein HMGBCG HMG-box containing 4 high-mobility group protein 2-like 1 HMG box domain containing 4 |
| Molecular Weight(MW) | 66 kDa |
| Cellular Localization | Nucleus. |
IHC
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HMG2L1 Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit instructionsand DAB staining.
IHC
Paraformaldehyde-fixed, paraffin embedded (Rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HMG2L1 Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit instructionsand DAB staining.| Application Notes | IHC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
