WB
Blocking buffer: 5% NFDM/TBST, Primary ab dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: C2C12, RAW264.7, EL4.IL-2, Protein loading quantity: 20 μg, Exposure time: 60 s.DB
Blocking buffer: 5% NFDM/TBST, Primary ab dilution: 1:2000,Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Immunogen peptide quantity: 1 ng, 4 ng, 16 ng Exposure time: 60 s.IHC
Tissue: Human breast cancer Section type: Formalin fixed & Paraffin - embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue)ICC/IF
Cell line: HeLa, Fixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:100 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Counter stain: Tubulin (Red).IP
IP of HeLa+ sodium lactate (100mM, 24hr) cells extracts IP ab incubation condition:,4°C overnight, 1:50 dilution WB primary ab incubation condition: PTM1406RM, room temperature 2h, 1:1000 dilution Secondary ab: Anti-Rabbit IgG for IP (HRP) Blocking buffer and concentration: 5% NFDM/TBST Diluting buffer and concentration: 5% NFDM/TBST Lane 1: 5% Input Lane 2: IP with PTM-1406RM Lane 3: IP with Rabbit monoclonal IgG Isotype Observed MW: 15 kDa Exposure time: 30 s.| Product Name | Anti-L-Lactyl-Histone H3 (Lys18) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DBICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:200-1:1000 ICC/IF:1:50-1:100 IP:1:50 DB:1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | H3K18lac |
|---|---|
| Molecular Weight(MW) | 15kDa |
| Function | Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. The Warburg effect, which originally described increased production of lactate in cancer, is associated with diverse cellular processes such as angiogenesis, hypoxia, polarization of macrophages and activation of T cells. This phenomenon is intimately linked to several diseases including neoplasia, sepsis and autoimmune diseases. Lactate, which is converted from pyruvate in tumour cells, is widely known as an energy source and metabolic by-product. Zhang et al. (Nature, 2019) reveal that histone lactylation is a previously unknown histone modification, derived from the cellular metabolite lactate. |
| Cellular Localization | Nucleus. |
WB
Blocking buffer: 5% NFDM/TBST, Primary ab dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: C2C12, RAW264.7, EL4.IL-2, Protein loading quantity: 20 μg, Exposure time: 60 s.
DB
Blocking buffer: 5% NFDM/TBST, Primary ab dilution: 1:2000,Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Immunogen peptide quantity: 1 ng, 4 ng, 16 ng Exposure time: 60 s.
IHC
Tissue: Human breast cancer Section type: Formalin fixed & Paraffin - embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue)
ICC/IF
Cell line: HeLa, Fixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:100 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Counter stain: Tubulin (Red).
IP
IP of HeLa+ sodium lactate (100mM, 24hr) cells extracts IP ab incubation condition:,4°C overnight, 1:50 dilution WB primary ab incubation condition: PTM1406RM, room temperature 2h, 1:1000 dilution Secondary ab: Anti-Rabbit IgG for IP (HRP) Blocking buffer and concentration: 5% NFDM/TBST Diluting buffer and concentration: 5% NFDM/TBST Lane 1: 5% Input Lane 2: IP with PTM-1406RM Lane 3: IP with Rabbit monoclonal IgG Isotype Observed MW: 15 kDa Exposure time: 30 s.| Application Notes | WB:1:500-1:2000 IHC:1:200-1:1000 ICC/IF:1:50-1:100 IP:1:50 DB:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
