WB
Western blot analysis of lysates from Mouse thymus using ARID5a Mouse mAb at 1:1000 dilution.
Secondary antibody:HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.