WB
Western blot analysis of lysates from MCF7 cells, using Pan Lactic acid-Lysine Rabbit pAb at 1:400 dilution. MCF7 cells were treated with Sodium lactate(100mM) for 24h. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.WB
Western blot analysis of lysates from NIH/3T3 cells, using Pan Lactic acid-Lysine Rabbit pAb at 1:400 dilution. NIH/3T3 cells were treated with Sodium lactate(100mM) for 24h. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit . Exposure time: 60s.WB
Western blot analysis of lysates from C6 cells, using Pan Lactic acid-Lysine Rabbit pAb at 1:400 dilution. C6 cells were treated with Sodium lactate(100mM) for 24h. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.| Product Name | Pan Lactic acid-Lysine Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Function | Histone lysine lactation (Kla) is a newly discovered histone modification that regulates gene expression in macrophages. In M1 macrophages, lactic acid is derived from incompletely oxidized glucose and then produces lactyl-CoA, which is transferred via acetyltransferase p300 to the lysine tail of the histone. This modification is abundant in gene promoter regions that lack acetylation and are associated with gene expression activation. |
|---|
WB
Western blot analysis of lysates from MCF7 cells, using Pan Lactic acid-Lysine Rabbit pAb at 1:400 dilution. MCF7 cells were treated with Sodium lactate(100mM) for 24h. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
WB
Western blot analysis of lysates from NIH/3T3 cells, using Pan Lactic acid-Lysine Rabbit pAb at 1:400 dilution. NIH/3T3 cells were treated with Sodium lactate(100mM) for 24h. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit . Exposure time: 60s.
WB
Western blot analysis of lysates from C6 cells, using Pan Lactic acid-Lysine Rabbit pAb at 1:400 dilution. C6 cells were treated with Sodium lactate(100mM) for 24h. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.| Application Notes | WB:1:500-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.09% sodium azide,50% glycerol,pH7.3. |
